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QuantaSep® 1000LX


*General QuantaSep Information: Specifications | FAQ | Presentation

QuantaSep® 1000LX is a unique integrated system that enables low flow methods development and high flow scale up in one machine. With its powerful Windows®-based software, it allows the user to automatically change buffers, collect fractions, and run gradients. The user can control each step in the purification based on UV, pH, conductivity, time, volume, air sensors or any external user-defined variable. All event, alarm, method, and chromatography data is logged for analysis or archiving. When methods development is complete, simply switch to the higher flow tubing to scale-up and make clinical product.

Hardware Features:

  • Two Systems in One: 0-100ml/min and 0- 1000ml/min
  • Interchangeable pump heads allow easy changeover from low to high flow system
  • Two complete sets of flow paths with valves and electronics that are easy to connect to.
QuantaSep® 1000LX is a fully integrated system designed to truly automate your purification process from development through production. It is effectively two systems in one. It has two complete set of valving and flow- path and can operate as a 0- 100 ml/ min system or by simply switching the pump- head it can become a 0- 1000 ml/min system. In either configuration this sanitary bench top gradient system enables you to automatically equilibrate, load, wash, elute and regenerate your chromatography columns. You can also control each step of the purification process based on UV, pH, conductivity, time, column volume, volume, air or % peak max. As the sensors monitor column outflow, the computer reads the information to automatically change buffers and collect fractions, stepping through the method as you programmed it. The computer logs all data such as events, alarms and chromatograms for analysis.

Fluid Handling

The entire flow path is sanitary and consists of polypropylene manifolds and reinforced silicone tubing for the high flowrate flow path and polyethylene tubing for the low flowrate flow path. All components are housed in a compact bench top system and use standard power. The salient features are labeled above in the Process Flow Diagram and referenced below:

(A) 12 selectable buffer ports –For each flow path there is a manifold of six solenoid actuated diaphragm valves on each pump which provide ample sample and buffer connections. Single piece construction minimizes hold up volume and makes sanitization easy.

(B) Two precision positive displacement rotary piston pumps – For each flow path either a 0-100ml/min or 0- 1000 ml/min pumps are used to provide accurate flow with low pulsation with turn down ratio of 100:1. Precision gradients are easily achieved between 5 -95%.

(C) In-line mixer with a pressure sensor

(D) Auto air eject - An "active air trap" consisting of an air sensor and three-way air ejection valve continuously detects and removes unwanted air before the column thus preventing loss of raw material and valuable production time.

(E) Multiple Columns and forward/reverse/bypass capability – For each flow path, the flow is directed to the column through a valve manifold, which can route flow to the column in a forward, reverse or bypass mode. Three columns steps or UF/DF can be run sequentially.

(F) Post column multi-mode flow cell- Consist of a single flow cell capable of UV dual-wavelength (280 and 254 nm) detection, pH, conductivity and temperature detection. Improves sensor response and synchronicity and minimizes hold up volume.

(G) 10 user selectable fraction ports - Fractions can be collected via a manifold compromising of 10 valves for each flow path. The components are mounted inside an enclosed stainless steel cabinet with a trough to which is attached a leak detector which detects and alarms in the event of a leak in a seal or fitting in the fluid path.

Controls Module

The controls module contains all power supplies, transformers, valve activation electronics, brainboards, sensor controls and circuit boards, alarm controls, sensor electronics including A/D and D/A converters and fuse assembly. The external chart recorder interface, status LED's, an emergency stop switch, cables, computer interface, fuse blocks, etc are all included.

Software Features

The Right Combination of Simplicity and Power

The simplicity of the QuantaSep® interactive graphical user interface gives the system its efficiency and power by making it easy to use and learn. The main screen below displays a flow diagram of the system with all the main components and their real time status quickly and easily. By simply clicking the mouse, you can open or close a valve, stop or start a pump, set new flow rates, collect fractions and perform other system operations all from your work station.

Intuitive Protocol Design

Programming is simple. Open the method editor by clicking on the icon on the tool bar. Then key in steps in the table, stepping through different buffers and changing buffer or fraction steps based on UV, conductivity, pH or air. Simply click on the gradient box and choose your gradient profile. Click on the event box and zero the UV baseline before you load your product or program in a pause before you start eluting your product!


The multi-levels of password protection restrict access to the system. For instance, an operator may have provision to run a method but not change the parameters; only the supervisor may be given that responsibility. In another instance, only the QA or metrology group may have access to setting calibration parameters. The security administrator in the software can enable all this and more.

CGMP Documentation and Data Analysis

All events manual or automatic (including deviations) are recorded to the batch log. Reports consisting of the method, buffers, all alarms and events, chromatogram data and analysis can be printed or archived as part of the batch log. The Instant Data Analysis helps do a quick check of an ongoing process against baseline data hence preventing possible losses.

Control and Power Module Contents

The control and power modules contain: All power supplies, sensor controls and circuit boards, alarm controls, sensor electronics including A/D and D/A converters, the external fraction collector interface, external chart recorder interface, external (e.g., user defined detector input) interface, digital display of monitor outputs, status LED’s, an emergency pause button, cables, computer interface, fuse blocks, etc.

Computer and Software

The system is controlled by a personal computer running a recent MicroSoft operating system.

Click here for Software Slide Show.


QuantaSep Family Details

QuantaSep® 100

QuantaSep® 1000

QuantaSep® 1000LX

QuantaSep® 1800

QuantaSep® 5000

QuantaSep® 10000

Custom QuantaSep®

QuantaSep® 15000

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