A Monolithic, Fast Flow Ion Exchange Media
for Efficient Purification from Crude Feed Streams
 |
Time is one of our most precious resources - to the
separation scientist
this means purifying the target biomolecule by the most direct, practical route -
SepraSorb® cellulose ion exchange media is designed to meet this challenge - allowing
rapid, low pressure primary purification in a fraction of the time taken by conventional
processes.
|
The Problem: Production Scale from Crude Feed Streams
Due to their resolution and binding capacity, beaded media are extensively used
in the laboratory for the isolation of pure proteins. While relatively problem free at
this scale, their use in production scale has been found inadequate. Low flow rates
(0.3 -1.0 m/min.) and high back pressures increase process time, inflate costs and lower
yields. Problems in packing, fouling, cross contamination , pre-treatment steps,
blockage of column frits and prefilters and CIP are costly and time consuming.
Susceptibility to shrinking and swelling causes channeling.
The Solution: SepraSorbâ
SepraSorbâ was developed specifically as an
alternative to the beaded matrices. It is a
cross-linked, sponge-like, regenerated cellulose material with a continuous,
interconnected, open pore (50-300 micron) structure (see photo above). This monolithic
matrix has readily accessible surfaces on to which the ion exchange functional groups
(DEAE, QM, CM & SE) are easily immobilized. Feed stream liquids actually flow through
the interconnecting pores of the continuous matrix, as opposed to around the beads as in
conventional media.
SepraSorbâ provides many advantages over beaded
media, in production scale. It can easily accommodate flow rates of 100 ml/min with
back pressures rarely exceeding 1 bar (14.5 psi). With SepraSorbâ
frits are unnecessary, eliminating the problems in fouling, cross contamination and
blockage. It is very easy to handle and configure avoiding cumbersome and time consuming
column packing. The matrix avoids channeling and is resistant to cracking. It doesn’t
shrink or swell in presence of strong alkali or acids and is amenable to CIP SepraSorbâ is able to withstand NaOH (up to 10 M), urea (up to 8M) and
Guanidine HCl (up to 6M) without loss of structural integrity. SepraSorbâ is competitively priced to warrant single process runs, which
eliminates CIP and cross contamination. It doesn’t foul or lose performance due to
air entrapment and it is biocompatible. The cross-linked interconnected structure avoids
the generation of fines, while allowing primary single step purification of the crudest,
particulated extracts from bacterial lysates, yeast homogenates, crude blood fractions,
fungal cultures and tissue supernatants.
By using SepraSorbâ it is now possible to move the
chromatography step up-stream in the processing of biomolecules. Clarification steps like
filtration, centrifugation and precipitation, which are invariably used prior to
performing chromatography on beaded matrices, may be by-passed. Scale up using SepraSorbâ can significantly simplify, cut processing costs and improve
overall productivity of the downstream process.
SepraSorbâ cartridges (10ml, 2.5cm x 2.0 cm) are
available in all four normal ion exchange chemistries. Information available on the
application of SepraSorbâ includes: fractionation of cheese
whey, isolation of cow milk proteins, purification of IgGs from tissue culture
supernatants and egg yolk, primary purification of membrane proteins, purification of
recombinant lysozymes from aspergillus niger and antibodies, purification of blood
plasma proteins, etc.
More Information
|
