|
| | ® Column Applications
| The
"IgG Purification Kit" based on SepraSorb® ion exchange
columns can purify antibodies from egg yolk faster and with greater
purity and recovery than traditional purification procedures. |
|
Reference:
Collin McKee, Scottish National Blood Transfusion Service, Edinburgh
|
|
^back
| Partial
Purification of an Over-expressed Recombinant Protein from an E.coli
Lysate |
Kd
78
30
12
|
|
|
1
2 3 4 |
|
Column:
SEPRASORB® CM
Flow Rate:
Application and elution by syringe
Feedstock:
E.coli cells sonicated, diluted 1:5 in 50 mM NaAc, pH 4.5
Elution:
50mM carbonate pH 10.6
Column
Loading: 5 ml feedstock (equivalent to 80 ml culture broth
grown to optimal density.)
|
12.5%
SDS-PAGE analysis:
Lane 1.
Low molecular weight markers
Lane 2.
E.coli homogenate
Lane
3. Breakthrough/ wash fraction
Lane
4. Elution (recombinant protein enrichment)
|
| SepraSorb®
Method |
Conventional
Method |
| Step |
Run
Time(h:m:s) |
Step |
Run
Time (h:m:s) |
| Column
Application |
00:20:00 |
Centrifugation |
01:00:00 |
|
Less
Time |
Precipitation |
02:00:00 |
| Dialysis |
14:00:00 |
|
|
|
^back
|
