Two Tank Method
The two-tank method of a gradient formation involves the
use of two buffer holding talks and 2 single speed pumps. Tank B is
initially 100% B, and Tank A is 100% A.
This assumes that mixing of A+B is instantaneous, but this will occur
if you use a sparger in the A+B tank.
If flow of B= ½ for of A+B then a continuous linear
gradient from 0-100% will result
If flow of B is greater, then gradient will be convex
If flow of B is less, then gradient will be concave
| Advantages |
Disadvantages |
| Can perform full 0-100%
gradients
Reduced cost due to single speed pump
Very good gradient accuracy |
Requires large tanks
Cannot perform step gradient
Cannot perform multi-step gradients
Cannot do on-line |
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